Академическое издательство «Гео»

Key words: Rhododendron schlippenbachii, in vitro propagation, zeatin, thidiazuron.

Rastitel'nyj Mir Aziatskoj Rossii (Plant Life of Asian Russia), 2015, № 4, p. 79-85

Conservation and propagation of Rhododendron schlippenbachii using biotechnological methods

Yu.G. Zaytseva, T.I. Novikova

Central Siberian Botanical Garden, SB RAS,
630090, Novosibirsk, Zolotodolinskaya str., 101, e-mail: ulianna_zaitseva@mail.ru

A protocol for R. schlippenbachii micropropagation from seeds revealed the high germinating capacity and
shallow type of dormancy was developed. The effect of cytokinins such as zeatin and thidiazuron (TDZ) on morphogenic potential of R. schlippenbachii seedling was tested. The highest regeneration rate (100 %) was achieved in the presence of 1.0 μM TDZ or 2.5 μM of zeatin in Anderson’s nutrient medium (AM). The direct organogenesis occurred both through activation of axillary meristems and differentiation of adventitious buds on explants hypocotyl. The maximum number of shoots per explant was obtained under 1.0 μM TDZ. The cultivation of regenerants on hormone-free AM allowed to obtain the elongated shoots and to overcome the anomalies caused by TDZ treatment. The largest percentage of rooting plants was achieved after pulse treatment with 148.0 μM indolyl-3-butyric acid for 4 hours followed by transferring to ex vitro conditions in mixture of peat and sand. The results could be included in the program for conservation of this rare polymorphic ornamental species and creation of new varieties on its basis.

Key words: Rhododendron schlippenbachii, in vitro propagation, zeatin, thidiazuron.