Peculiarities of pollination of flowers of Hedysarum theinum Krasnob. ( Fabaceae ) are considered. The interrelationship between the polypeptide variation and pattern of pollination was analyzed in 13 populations of this species. Cross pollination was revealed in 7 populations, self-pollination — in 2 populations and both types of pollina-tion — in 4 populations. Thus the method of electrophoresis of seed polypeptides allows forecasting a predominant type of pollination in H. theinum populations.Key words: polypeptides, electrophoretic spectra, Hedysarum theinum, Fabacee , self-pollination, antecology.